The present disclosure provides an organic light-emitting display panel, a method of manufacturing the organic light-emitting display panel, and a display apparatus. The organic light-emitting display panel includes: a substrate having an opening passing through the substrate; a pixel array disposed on the substrate and including a plurality of pixels; an isolation part disposed between the plurality of pixels and the opening and surrounding the opening, wherein the isolation part includes: a first layer disposed on the substrate, wherein the first layer includes a first portion and a second portion which are sequentially stacked in a direction away from the substrate, and an orthogonal projection of the first portion on the substrate falls within an orthogonal projection of the second portion on the substrate; and a second layer disposed on a surface of the first layer away from the substrate.