Provided are a display substrate, a preparation method thereof, a method for bonding a display panel, and a display apparatus. The display substrate includes a display region and a bezel region, the display region includes multiple corner regions and the corner regions include a corner display region and a blank corner cutting region which are alternately disposed. The corner display region includes a composite wiring layer disposed on a base substrate, a drive structure layer disposed on the composite wiring layer and light-emitting elements disposed on the drive structure layer. The composite wiring layer includes a first wiring layer, a second wiring layer and a first insulating layer disposed between the first wiring layer and the second wiring layer. The drive structure layer includes a pixel drive circuit and multiple connection electrodes. The first wiring layer includes a gate line, and the second wiring layer includes a data line.