A display substrate includes a display region, a non-display region at a periphery of the display region, pixel units in the display region and arranged in an array, gate lines extending in a row direction of the array, and data lines extending in a column direction of the array. Each row of pixel units is provided with at least one gate line, and each gate line is coupled to at least a part of the pixel units in a corresponding row. Each column of pixel units is provided with at least two data lines, and each data line is coupled to a part of the pixel units in a corresponding column. Each pixel unit is coupled to one gate line and one data line, and two pixel units in a same column and in two adjacent rows are coupled to different data lines, respectively.