A photoelectric conversion apparatus in one aspect of the present disclosure includes a first semiconductor region of a first conductivity type, a second semiconductor region of the first conductivity type, a third semiconductor region of the first conductivity type, a fourth semiconductor region of a second conductivity type in which a distance from a first surface being greater than a distance from the substrate to the third semiconductor region, a first isolation portion disposed between the first semiconductor region and the second semiconductor region, a microlens commonly disposed in the first semiconductor region and the second semiconductor region, and a fifth semiconductor region of the second conductivity type disposed between the first isolation portion and the fourth semiconductor region. The third semiconductor region is disposed between the fourth semiconductor region and the fifth semiconductor region.