Provided is a detection circuit and a driving method thereof, and a display panel. The detection circuit includes: a plurality of first detection circuits, a plurality of second detection circuits, and a plurality of third detection circuits. The first detection circuit is connected to a first pin, a first control signal terminal, a first detection signal terminal, and a first gate line corresponding to the first detection circuit; the second detection circuit is connected to a second pin, a second control signal terminal, a second detection signal terminal, and a second gate line corresponding to the second detection circuit; the third detection circuit is connected to a first data line corresponding to the third detection circuit, a sensing signal line corresponding to the third detection circuit, a third detection signal terminal, and a third control signal terminal.