A detection method of a pixel circuit, a driving method of a display panel, and a display device are disclosed. The pixel circuit includes a driving transistor; and the detection method of the pixel circuit includes: in the first charge cycle, applying a first data voltage to a gate electrode of the driving transistor, acquiring a first sensing voltage at a first electrode of the driving transistor within the first duration after the application of the first data voltage and before the driving transistor is switched off, and determining whether the first sensing voltage is equal to reference sensing voltage.