An image sensing circuit includes a plurality of readout circuits and a pre-charge control circuit. Each of the readout circuits is coupled to a sensing line among a plurality of sensing lines and configured to receive a sensing voltage among a plurality of sensing voltages through the corresponding sensing line, wherein at least one of the readout circuits is further configured to receive at least one first pre-scan voltage through the corresponding sensing line prior to reception of the sensing voltage. The pre-charge control circuit, coupled to the readout circuits, is configured to perform a plurality of steps. The steps include receiving the at least one first pre-scan voltage from the at least one readout circuit; calculating a first pre-charge voltage according to the at least one first pre-scan voltage; and pre-charging the sensing lines to the first pre-charge voltage before the readout circuits receive the sensing voltages.