Provided is an anti-islanding protection system. The system is applied to a low-voltage distributed generation resource (DGR) and includes a box, a reverse power protector, a protection module and an output controller. The reverse power protector has an end connected to a first current transformer and has another end connected to the output controller. The reverse power protector is configured to provide reverse power protection for the low-voltage DGR. The output controller has an end connected to the protection module and the reverse power protector and has another end connected to a grid-connection switch of the low-voltage DGR. The output controller is configured to control the grid-connection switch to turn off when reserve power is detected. The protection module has an end connected to a second current transformer and has another end connected to the output controller. The protection module is configured to provide low-frequency protection, over-frequency protection, over-voltage protection and low-voltage protection for the low-voltage DGR.