A display substrate, a manufacturing method thereof, and a display device are provided. The display substrate includes: a base substrate including a main portion and an edge portion located on at least one side of the main portion; a first pixel located on the edge portion and including a first light-emitting surface; and an angle-adjusting portion located between the first pixel and the edge portion and configured to provide an included angle between the first light-emitting surface and the edge portion at a position where the first pixel is located, the included angle being not zero.