Pixel circuit and display substrate and driving methods, and display apparatus are provided. Pixel circuit includes: driving resetting sub-circuit to input voltage at first initial voltage terminal to gate of driving transistor in driving sub-circuit under control of first resetting signal terminal; writing compensation sub-circuit to input data voltage to driving sub-circuit and compensate driving sub-circuit under control of scanning signal terminal in writing compensation phase, and input reference voltage output at data voltage terminal to driving sub-circuit in blanking phase, so driving transistor in On-Bias state; light-emitting resetting sub-circuit to input voltage at first initial voltage terminal to light-emitting device to reset light-emitting device under control of scanning signal terminal; and light-emitting enabling sub-circuit to provide voltage at first power supply voltage terminal to driving sub-circuit and connect driving sub-circuit to light-emitting device under control of enabling signal terminal; and driving sub-circuit to provide driving current to light-emitting device.