A cell culture bag is provided, comprising a framed porous inner fluoropolymer bag that allows for growing and harvesting cells and an outer fluoropolymer bag that allows removal and replenishing feed media without loss of cells. In one embodiment, a bag comprising an outer bag and an inner bag is presented. The outer bag comprises a gas permeable porous film having a first port and a second port attached thereto and the inner bag comprises a gas permeable porous film having a third port attached thereto that passes through the outer bag to the inner bag. The inner bag has larger pore size than the outer bag to allow movement of media between the outer bag and the inner bag.