发明授权
- 专利标题: Methods and compositions for enriching compositions for polymerase enzyme complexes
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申请号: US15618535申请日: 2017-06-09
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公开(公告)号: US10544457B2公开(公告)日: 2020-01-28
- 发明人: Erik Miller , Keith Bjornson , Kristofor Nyquist , Satwik Kamtekar
- 申请人: Pacific Biosciences of California, Inc.
- 申请人地址: US CA Menlo Park
- 专利权人: Pacific Biosciences of California, Inc.
- 当前专利权人: Pacific Biosciences of California, Inc.
- 当前专利权人地址: US CA Menlo Park
- 代理商 Monicia Elrod-Erickson
- 主分类号: C12Q1/6874
- IPC分类号: C12Q1/6874 ; C12N9/96 ; C12N9/12
摘要:
The present invention provides methods, compositions, and systems for enriching compositions for polymerase enzyme complexes. In particular, the methods, compositions, and systems of the present invention remove free polymerases from the compositions using one or more purification steps, including protease treatment, thus enriching the compositions for polymerases complexed with a template nucleic acid.
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