A sealed fluid transfer device and a sealed fluid transfer method includes a first container puncture apparatus, a second container puncture apparatus and a fluid transfer apparatus, wherein the fluid transfer apparatus includes a first container puncture adapter, a second container puncture adapter and a pressure supply unit; the first container puncture adapter includes a sealing element fixed seat, a guided outer casing, a pipeline fixed seat, a first fluid pipeline and a second fluid pipeline; when the sealing element fixed seat is connected with the first container puncture apparatus, a first sealing element is firstly tightly connected with a second sealing element, the first fluid pipeline and the second fluid pipeline respectively pass through the two sealing elements and then communicate with the first container; after being firstly used, the first fluid pipeline and the second fluid pipeline are always in no contact with the exterior.