Disclosed are a method for multi-user transmission and reception in a wireless communication system and a device for same. More particularly, a method for performing multi-user (MU) transmission by a station (STA) device in a wireless communication system comprises the steps of: generating a high efficiency-long training field (HE-LTF) sequence in a frequency domain in accordance with an MU transmission bandwidth; and transmitting a physical protocol data unit (PPDU) which comprises one or more symbols to which the HE-LTF sequence is mapped, wherein the HE-LTF sequence can be generated by multiplying one row of a P matrix to a length unit of a row of the P matrix in a predetermined sequence.