The present invention relates to a particle manipulation method to disperse magnetic particles 70 in a liquid 35 filling up a tube container 10, wherein a circumferential direction moving step to move the magnetic particles 70 along the circumferential direction of the container 10 in the liquid 35 and in a radial direction moving step to move the magnetic particles 70 as crossing the radial direction of the container 10 in the liquid 35 are implemented repeatedly. Such manipulations can be achieved by combining rotation of the container and gravity force and magnetic field manipulations.