The present application discloses a display substrate having a first area for bonding a driver integrated circuit. The display substrate includes a base substrate; an overcoat layer on the base substrate in a second area of the display substrate; the overcoat layer having an interface portion in an area abutting the first area, the interface portion having a plurality of teeth and a plurality of slits, two adjacent teeth being spaced apart by a slit; and a conductive line layer on a side of the overcoat layer proximal to the base substrate, having a plurality of conductive lines extending from the second area to the first area; each of the plurality of conductive lines electrically connected to a signal line in the second area.