Holographic microscope and data processing method for high-resolution hologram image
摘要:
The present invention can realize both a transmission type and a reflection type, and provides a holographic microscope which can exceed the resolution of the conventional optical microscope, a hologram data acquisition method for a high-resolution image, and a high-resolution hologram image reconstruction method. In-line spherical wave reference light (L) is recorded in a hologram (ILR) using spherical wave reference light (R), and an object light (Oj) and an illumination light (Qj) are recorded in a hologram (IjOQR) using a spherical wave reference light (R) by illuminating the object with an illumination light (Qj, j=1, . . . , N) which is changed its incident direction. From those holograms, a hologram (JjOQL), from which the component of the reference light (R) is removed, is generated, and from the hologram, a light wave (hj) is generated. A light wave (cj) of the illumination light (Qj) is separated from the light wave (hj), and using its phase component (ξj=cj/|cj|), a phase adjustment reconstruction light wave is derived and added up as (HP=Σhj/ξj), and an object image (SP=|HP|2) is reconstructed.
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