Provided is a vial rubber stopper using multiple types of rubber to prevent the vial rubber stopper from being scraped and from sticking to a presser plate. The vial rubber stopper includes: a cap portion having a flange; a piercing area formed to have a recess at a top surface of the cap portion and configured to traverse longitudinally the cap portion in an axial direction from the recess to a bottom surface of the cap portion; and a leg portion formed on the bottom surface of the cap portion in a way of extending downward and located outside the piercing area with respect to an axial center of the cap portion. The piercing area is formed of rubber having rubber hardness from 20 to 35, and the top surface other than the recess of the cap portion is formed of rubber having rubber hardness from 58 to 90.