The utility model discloses an adhesive tensile shear strength sample preparation tool. Sample preparation positioning plate, a plurality of first positioning grooves and second positioning grooves coaxial with the first positioning grooves are linearly formed in the sample preparation positioning plate; a first rectangular groove communicated with the first positioning groove and a second rectangular groove communicated with the second positioning groove are respectively formed in the sample preparation positioning plate along the length direction; the first rectangular groove and the first positioning groove are perpendicular to each other and are arranged in the same plane, the second rectangular groove and the second positioning groove are perpendicular to each other and are arranged in the same plane, and the first rectangular groove and the second rectangular groove are arranged in a vertically staggered mode. A first demolding area is formed in the first rectangular groove, anda second demolding area is formed in the second rectangular groove. According to the utility model, the parallelism and the coaxiality of the first shearing sheet and the second shearing sheet can beensured to be consistent, the overlapping width and the thickness of an adhesive layer are ensured to be consistent, and redundant adhesives in the sample preparation process can be rapidly cleaned onsite.