The utility model discloses pretreatment equipment for detection. The pretreatment equipment comprises a base, the kit is arranged on the base, and an extracting solution bin, a magnetic bead bin andan elution bin are arranged on the kit; a concentration device; the liquid adding device is used for adding reconstitution fluid; the magnetic separation device is used for adsorbing magnetic beads from the magnetic bead bin, adsorbing a target compound by using the magnetic beads and eluting the magnetic beads; the moving device is used for adjusting the relative positions of the concentration device, the sample adding device, the magnetic separation device and the kit; and the control device is respectively connected with the concentration device, the liquid adding device, the magnetic separation device and the moving device. According to the utility model, an extracting solution can be concentrated in a solvent evaporation manner, a target compound in the extracting solution can be separated and purified by utilizing the magnetic beads through the magnetic separation device, meanwhile, the functions of concentration and separation and purification are achieved, the whole process isautomated through equipment, and the operation is convenient.