The utility model provides a simulation scalpel space positioning device. The device is composed of movable arms S1, S2 and S3, a simulation scalpel S4, universal rotating shafts S5, S6, S7 and S8 anda fixed seat S9. Wherein the movable arms S1, S2 and S3 are connected through a universal rotating shaft, the movable arms S1, S2 and S3 are connected with the simulation scalpel S4 through universalrotating shafts, a posture detection module is arranged in each movable arm, and a posture detection module and a calibration key are arranged in the simulation scalpel S4; the universal rotating shaft can perform three-dimensional space large-angle rotation around the axis; the posture detection module on each movable arm is used for capturing the posture of the movable arm in the moving process; the posture detection module on the simulation scalpel S4 captures the posture of the simulation scalpel S4 in the moving process, the function of positioning the virtual scalpel in virtual realityis achieved in combination with the movable arm and the length of the simulation scalpel S4, and the requirement for positioning surgical objects at different angles in virtual reality simulation surgical operation is met.