The utility model provides an enzymolysis ultrafiltration device for a sea cucumber and donkey-hide gelatin polypeptide nutritional product and a production system for the sea cucumber and donkey-hidegelatin polypeptide nutritional product, and relates to the technical field of nutritional health-care products. The enzymolysis ultrafiltration device comprises an enzymolysis tank body, an ultrafiltration membrane, a heating device and a control device; a feed liquid inlet and a hydrolase inlet are formed in the upper part of the enzymolysis tank body; the ultrafiltration membrane is arranged at the lower part of the enzymolysis tank body; the heating device is arranged on the side wall of the enzymolysis tank body and is electrically connected with the control device through a wire. According to the enzymolysis ultrafiltration device disclosed by the utility model, the ultrafiltration purification process can be completed while enzymolysis is carried out; the method has the advantagesof short time, high efficiency, convenience and quickness, and effectively alleviates the problems that in the existing polypeptide production technology, the enzymolysis and ultrafiltration processesneed to be separately carried out in different equipment, the production process is complex, the process is tedious, the occupied area of the equipment is large, and the industrial application is notfacilitated.