The utility model discloses a reagent card bearing component of in-vitro diagnostic equipment, which comprises a guide groove with a U-shaped longitudinal section, an opening with a quadrilateral cross section is formed in the bottom surface of the guide groove, and one end of the opening extends to the end part of the guide groove; A bearing piece for bearing a reagent card is arranged in the groove space of the guide groove; the bearing piece comprises a bearing piece upper part and a bearing piece lower part which are of an integrated structure, the bearing piece upper part comprises a first U-shaped groove and a second U-shaped groove which are vertically distributed, and the first U-shaped groove and the second U-shaped groove are symmetrically distributed around the central axis surface where the center of the reagent card is located; The lower part of the bearing part is an I-shaped part; and the middle part of the I-shaped part is inserted into the opening of the guide groove.According to the technical scheme, the structural shapes of the upper portion and the lower portion of the bearing part are improved, the bearing part can be tightly matched with the structural shapeof the U-shaped guide groove, the guide groove is of a U-shaped structure, the bottom face of the guide groove is provided with the opening, and therefore the bearing part can be installed and placedintegrally.