The utility model discloses a device suitable for luteolin separation and purification. Including chromatography column, stock solution device, receiving vessel, mount, pressure device, stock solutiondevice and chromatography column are fixed in the mount respectively, stock solution device upper portion is equipped with the opening, and the lower part is equipped with the export, and the exit linkage chromatography column of pressure device, the lower part of stock solution device is connected to its upper portion opening when using, the upper end of chromatography column is provided with the opening, and the bottom of chromatography column is provided with the export, and the exit of chromatography column is provided with control switch, and the export below of chromatography column sets up receiving vessel, the chromatography column traditional chinese clothing is filled with luteolin molecular engram polymer microballon pillar and filtering material. For current box -packed the putting of solid -phase extraction, the utility model discloses simple structure, with low costs, efficient, molecular imprinted polymer's the volume of loading is bigger, and to operate resveratrol purification volume big at every turn, and the actual small -size industrial production's of simulation process through gradient elution's mode, obtains the higher luteolin product of a large amount of purity, can be applied to the luteolin separation and purification well.