The utility model relates to a micro-fluidic chip which comprises a chip substrate and two films, wherein the two films cover the upper surface and the lower surface of the chip substrate; a sample feeding hole, a plurality of liquid runners and a plurality of gas outlets are formed in the chip substrate; the sample feeding hole is connected with the plurality of liquid runners; the gas outlets are formed in the tail ends of the liquid runners; the sample feeding hole vertically passes through the chip substrate; each liquid runner consists of a guide groove, a transfer groove, a microfluid channel and a reaction tank; the guide groove is formed in the lower surface of the chip substrate; one end of the guide groove is communicated with the bottom end of the sample feeding hole; the other end of the guide groove is communicated with the lower end of the transfer groove; the transfer groove vertically penetrates through the chip substrate; the upper end of the transfer groove is communicated with one end of the microfluid channel; the microfluid channel is horizontally arranged on the upper surface of the chip substrate; the lower part of the microfluid channel is communicated with the reaction tank; the tail end of the microfluid channel is communicated with the gas outlet holes. The micro-fluidic chip can be widely applied to the technical field of microfluid control.