The utility model discloses a multiple function test tube frame, comprising an upper face plate, a lower face plate and a supporting plate. The upper face plate and the lower face plate are fixedly connected with the supporting plate, one side of the upper face plate is laterally provided with a plurality of rows of holes, the other side of the upper face plate is laterally provided with a plurality of concave grooves, and the opening end of the concave groove is provided with a boss raised upwards. The test tube frame has simple structure and multiple functions. Test tubes, pipets and transfer pipets can be placed in the holes, and measuring cylinders (cups) can be placed in the concave grooves to keep the clean storage. The utility model has accelerating function to the ladle analysis of copper metallurgy and the emergency assay in hospitals.