The utility model relates to a quantitative anoectochilus roxburghii culture liquid filling device. The device comprises a filling platform and is characterized in that a temporary culture liquid storage tank is arranged above the filling platform, a movable bench capable of rising upwards and falling downwards is arranged at the lower part of the temporary culture liquid storage tank, a plurality of longitudinal guide pipes for filling culture liquid are connected to the movable bench, the upper ends of the longitudinal guide pipes extend in the temporary culture liquid storage tank, and sealing rings are respectively arranged on the longitudinal guide pipes and located at the inner and outer sides of the temporary culture liquid storage tank. According to the device, the structure is simple, the operation is convenient and fast, the filling volume is stable, and the filling efficiency is high, so that the needs of production can be met effectively.