The utility model provides a test dissecting needle with a protective cover and a temperature display. The protective cover is configured outside the needle body of the test dissecting needle, and is provided with a hanging ring, and the tail part of the handle of the needle body is provided with the temperature display. In the utility model, by the protective cover, when the dissecting needle is put away, a human body can be prevented from being accidentally hurt, and the infection of pathogenic bacteria can be prevented; and the temperature display is beneficial to mastering the environment temperature in a test and the humidity condition of a test sample.