The pre-treatment process of fluorescence-free bleached towel includes the working procedures of accepting fabric, refining, bleaching, dewatering and stoving. The working procedures of refining and bleaching includes the following steps: circulating the towel in the refining liquid with biological enzyme in 1-2 g/L and surfactant in 0.3-0.8 g/L at 40-60 deg.c for 30-50 min, and conventional water washing; circulating in bath with hydrogen peroxide in 2 g/L and hydrogen peroxide stabilizer in 1 g/L and of pH 10-11 at 90-98 deg.c for 20-40 min; and high temperature water washing at over 95 deg.c and cold water washing. The process is simple and reasonable, and can eliminate commensals from pure cotton towel and endow towel with natural whiteness and excellent wearability.