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发明授权
CN1974600B 抗氟甲喹的单克隆抗体、其制备方法及其应用 失效 - 权利终止

抗氟甲喹的单克隆抗体、其制备方法及其应用
摘要：
本发明提供了一种抗氟甲喹单克隆抗体，其由小鼠杂交瘤细胞株2D12CGMCC No.1830产生的。其制备方法为采用碳二亚胺法和混合酸酐法将氟甲喹和载体蛋白BSA、OVA偶联，合成人工免疫原FLU-BSA和包被原FLU-OVA；用合成的免疫原FLU-BSA免疫Balb/c小鼠，将免疫脾细胞和SP2/0骨髓瘤细胞融合，用包被原FLU-OVA建立间接竞争ELISA方法筛选能分泌特异性抗体的细胞株。获得的细胞株的细胞培养上清效价在1∶4000以上，IC50为55ng/ml；细胞上清液对恩诺沙星、二氟沙星、氧氟沙星、噁喹酸和诺氟沙星的交叉反应率小于或等于1％。利用细胞株制备的腹水，纯化后效价可以达到1∶640,000以上。本发明的抗体可用于研制氟甲喹的酶联免疫试剂盒。
摘要（英）：

The present invention provides one kind of flumequine resisting onoclonal antibody generated by mouse hybridoma strain 2D12 CGMCC No. 1830. Its preparation process includes the following steps: coupling flumequine and carrier protein BSA and OVA via carbodiimide process and mixed acid process to synthesize artificial immunogen FLU-BSA and coating antigen FLU-OVA; immunizing Balb/c mouse with the synthesized immunogen FLU-BSA to fuse spleen cell and SP2/0 myeloma cell, and establishing and screening cell strain capable of secreting specific antibody with coating antigen FLU-OVA through an indirect competition ELISA process. The cell culture supernatant of the cell strain has potency over1 to 4000, IC50 of 55ng/ml, and cross reacting rate not greater than 1 % on enrofloxacin, difloxicin, ofloxicin, oxolinic acid norfloxicin. The ascites prepared with the cell strain and purified has potency over 1 to 640,000. The antibody may be used in developing flumequine enzyme-linked immune kit.

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