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发明授权
CN1966683B 表达新型肿瘤抑制基因p53的重组腺病毒 失效 - 权利终止 转让

表达新型肿瘤抑制基因p53的重组腺病毒
摘要：
本发明为一种表达新型肿瘤抑制基因p53的重组腺病毒的表达结构，其主要特征为1)野生型P53抑癌基因的第72位氨基酸由脯氨酸突变成为精氨酸，其他氨基酸组成及其排列顺序不变；2)该P53抑癌基因在其N-末端以内切酶Nhel和C-末端以内切酶BamH1片段拼接于穿梭质粒pDC315超强启动子mMCV的下游；和3)重组的腺病毒载体为以Ad35纤突结取代Ad5型腺病毒的纤突结的复制缺陷型腺病毒载体。该新型p53抑癌基因腺病毒具有感染力更强、感染组织/细胞谱更广、表达新型p53抑癌基因更强、其产物具有更强细胞凋亡生物功能。
摘要（英）：

The invention discloses the expression structure of a recombinant adenovirus p53 for expressing anti-tumor gene, characterized in: (1) the 72nd amino acid of the wild p53 anti-tumor gene changes frompraline to arginine, other amino acids components and their arranging sequences do not change, (2) splicing the p53 anti-cancer gene to the shuttle vector pDC315 strong promoter mMCV downstream at itsN-terminal with incision enzyme Nhe1 and C-terminal with incision enzyme BamH1 fragment, and (3) the recombinant adenovirus is replication defective adenovirus displacing junction of adenovirus Ad5 with Ad35 junction. The new p53 anti-cancer gene adenovirus has strong infectivity, broad infection tissue/cell, strong expression of p53 anti-cancer gene, and product with strong apoptosis.

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