The provided low-temperature disinsection equipment comprises: a cold store room (20) for the being treated target (1), a normal temperature room (3), and a conveying room (4), wherein the opposite treatment method comprises: using take-in tool (1a) to insert a temperature sensor into target, conveying the target into (20) by conveying machine (22, 23, 26); wireless sending the detection signal of target inside to a control device sensor, conveying the treated target with machine (26, 23, 24, 36) into room (3) to recover normal temperature.