The invention discloses a mycobecterium tuberculosis vaccine series medicaments and its use, wherein the series medicaments consist of matched mycobacterium tuberculosis vaccine and Calmette, the mycobacterium tuberculosis vaccine being nucleic acid sequences obtained through subjecting Ag85B, MPT-83 and MPT-64 to fusion or deletion of partial sequence or partial mutation with other nucleic acidscapable of encoding nucleic acid sequences having the same reactive protein as Ag85B, MPT-83 and MPT-64, they are cloned into eucaryon expressed carrying agent, thus obtaining at least two of the prepared expression carriers for combination.