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发明公开
CN1821407A 一种转基因番茄高效稳定表达体系 失效 - 权利终止

一种转基因番茄高效稳定表达体系
摘要：
本发明克隆获得了我国优良纯种番茄ZhongshuNo.5果实特异性启动子E82.2和E81.1基因，分别用番茄果实特异性E82.2启动子以及E35S－E81.1复合式双启动子驱动疫苗分子并与NOS终止子构成植物表达操纵子单元，导入根癌农杆菌LBA4404获得工程菌；通过优化包括培养基等试剂配方、技术方案以及操作流程建立了番茄高频再生体系、番茄基因转化、抗性筛选、植株培育及其分子鉴定等完整的技术体系，获得能高效稳定表达的疫苗分子重组蛋白及其番茄转化株系(部分植株目的蛋白的表达量可达番茄组织可溶性总蛋白10％以上，且经过蛋白纯化、Western blot、ELISA分析表明所获重组蛋白具有良好的免疫学活性)。本发明构建了可用于外源疫苗分子及药用蛋白生产的番茄高效稳定表达体系技术平台，为深入研发番茄口服疫苗及其药用蛋白提供了良好的技术平台。
摘要（英）：

Through cloning to obtain the specific fruit promoters E82.2 and E81.1 gene of excellent pure tomato breed Zhongshu No.5, driving the vaccine molecule with the promoter E82.2 and the compound double promoter E35S-E81.1 and constituting plant expression operon unit with NOS terminator, and introducing to Agrobacterium tumefaciens LBA4404, engineering bacterium is obtained. By means of optimizing the complete technological system, recombinant vaccine molecule protein with efficient and stable expression and its tomato transforming strain are obtained. The present invention constitutes technological platform of efficient and stable tomato expression for exogenous vaccine molecule and medicinal protein production and provides excellent technological platform for developing oral tomato vaccine and its medicinal protein.

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