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发明授权
CN1814755B 一种高温中性蛋白酶及其制备方法 失效 - 权利终止

一种高温中性蛋白酶及其制备方法
摘要：
本发明公开一种地衣芽孢杆菌(Bacillus Licheniformis CGMCC N00800)生产高温中性蛋白酶及其制备方法。通过对高温中性蛋白酶的分离、纯化及分子生物学研究，可获得高温中性蛋白酶的结构基因。该基因片段大小为1129个碱基，表达377个氨基酸。并据此设计了DNA引物，同时克隆出该基因的全基因序列，确定酶蛋白的纯化方法及基因克隆的方法，并对表达载体进行了选择，使该基因与pET28a进行连接，并在大肠杆菌BL21中进行了表达。在获得基因后，并克隆到表达载体上，可能获得高效表达的基因工程菌株，再进行发酵生产，能显著提高酶产量的有效途径。利用该技术生产的产品将使我国洗涤剂工业、毛皮工业、制革工业、丝纺工业、药品及食品工业产生一次变革，可减少生产过程中对环境的污染，提高生产效率和产品质量。无论直接经济效益或社会意义都是很大的。
摘要（英）：

This invention discloses a high temperature neutral proteinase generated by Bacillus Licheniformis CGMCC NO0800) and its preparation method, in which, the structure gene of high temperature neutral proteinase can be got by separating, purifying and studying, said gene includes 1129 alkali bases to express 377 amino acids so as to design DNA primer and clone the whole series of the gene to determine the purification method for enzyme protein and its gene clone method and selects the expressing carrier and said gene is connected with pET 28a to be expressed in colibacillus. After getting the gene, it is cloned to the expression carrier to obtain high efficienct expressed gene engineering strains to be fermented to increase the enzyme production obviously.

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