The moisture-permeating anti-seepage high strength non-woven fabric is used in making operation suit, protecting suit, isolating suit and other disposable products. It consists of outer non-woven fabric layer and inner microporous film layer adhered together with hot melt adhesive, with the hot melt adhesive being in micro point distribution on the non-woven fabric layer. The preparation process includes spraying hot melt adhesive fog with a spraying gun on the non-woven fabric layer, covering with the microporous film and final pressurized forming to make the composite non-woven fabric. The outer non-woven fabric layer can ensure the enough strength and the inner microporous film layer ensures the moisture-permeating and anti-seepage property to block alcohol, water, plasma, etc and the hot melt adhesive in micro point distribution serves for adhesion and has no influence on the moisture-permeating and anti-seepage property.