The invention provides an industrialized production method for full-suspension culture of a Seneca valley virus by adopting a cell BHK-21. The method comprises the steps of cell thawing, cell passage,toxin inoculating and toxin collecting, wherein reaction parameters and conditions of each process are defined. According to the method, a bioreactor serum-free full-suspension culture process is used, and the large-scale propagation culture of the Seneca valley virus is achieved; and compared with an adherence cell culture process, the process has the advantages of simple and convenient process,stable toxin production effect, low production cost and the like, so that a foundation is settled for later-stage large-scale production of Seneca valley virus vaccines.