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发明公开
CN110810246A 一种红芽大戟繁育方法 审中-实审

一种红芽大戟繁育方法
摘要：
本发明提供了一种红芽大戟繁育方法，包括以下步骤：1)外植体灭菌：取红芽大戟植株茎秆，酒精消毒，无菌水清洗，次氯酸钠溶液消毒，无菌水冲洗；2)愈伤组织诱导培养：茎秆剪成茎段，诱导培养基为：MS+0.5-1.5mg/L KT+0.3-0.6mg/L 6-BA+20g/L蔗糖+7.5g/L琼脂；3)愈伤组织分化培养：分化培养基为：MS+0.1-0.5mg/L KT+0.5-1mg/L 6-BA+20g/L蔗糖+7.5g/L琼脂；4)继代培养：MS+0.5-1mg/L NAA+1-2mg/L 6-BA+0.5-1mg/L 2,4-D+25g/L蔗糖+7.5g/L琼脂；5)生根培养：生根培养的培养基为：1/2MS+0.3-0.6mg/L IBA+20g/L蔗糖+5g/L琼脂；6)移栽；本发明红芽大戟繁育方法可快速繁殖出品质优异的红芽大戟幼苗，并可降低红芽大戟的繁育成本。
摘要（英）：

The invention provides a knoxia breeding method, which includes the following steps: (1) sterilizing an explant: taking a stem of a knoxia plant, disinfecting with alcohol, washing with sterile water,disinfecting with a sodium hypochlorite solution, and washing with sterile water; (2) callus induction culture: cutting the stem into stem segments, wherein the induction medium is MS + 0.5-1.5mg/L of KT + 0.3-0.6mg/L of of 6-BA + 20g/L of sucrose + 7.5g/L of agar; (3) callus differentiation culture: wherein the differentiation medium is MS + 0.1-0.5mg/L of KT + 0.5-1mg/L of 6-BA + 20g/L of sucrose + 7.5g/L of agar; (4) subculture: wherein the cultrue medium is MS + 0.5-1mg/L of NAA + 1-2mg/L of 6-BA + 0.5-1mg/L of 2,4-D + 25g/L of sucrose + 7.5g/L of agar; (5) rooting culture: wherein the culture medium for rooting culture is 1/2 MS + 0.3-0.6mg/L of IBA + 20g/L of sucrose + 5g/L of agar; and (6) transplanting. The knoxia breeding method can quickly breed knoxia seedlings with excellent quality and can reduce the breeding cost of the knoxia.

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