The invention provides a culture medium for tissue culture of hedysarum scoparium. The culture medium comprises a primary induction medium, a subculture medium and a rooting medium. Growth points on young shoots of the hedysarum scoparium can be used as explants, and primary induction culture, subculturing, rooting culture and transplanting are carried out after sterilization. The culture medium has the advantages that the selected optimal culture medium formula and combinations thereof for each growth stage of the whole tissue culture process of the hedysarum scoparium can fully meet nutrition requirements and growth and development requirements in each stage of the hedysarum scoparium, the whole process of tissue culture and expanding propagation of the hedysarum scoparium is promoted, the successful implementation of the tissue culture of the hedysarum scoparium can be ensured, and a strong support is provided for large-scale production of the hedysarum scoparium. The culture mediumhas the advantages of simple formula, low cost and good application prospect.