The invention discloses a detection method of fatigue degrees of programmers. The detection method of the fatigue degrees of the programmers comprises the following steps: step 1, monitoring change ofa programmer's head movement, as well as output of a keyboard and a mouse, in a period from a moment T to a moment T+1 by a sensor so as to obtain data, assembling all data, and sending the assembleddata to a processor; step 2, weighting a classifier according to the change of the programmer's head movement as well as the output of the keyboard and the mouse, and classifying possible states of the programmer so as to obtain the most probable state SK and machine probability PK; step 3, applying a screen pop-up and a warning tone testing signal at the moment T+1; step 4, monitoring change ofthe programmer's head movement, as well as output of the keyboard and the mouse, in a period from the moment T+1 to a moment T+2 by the sensor so as to obtain data, assembling all data, and sending the assembled data to the processor; and step 5, carrying out calculation by adopting a binomial distribution formula, taking the probable machine probability PK obtained in the step 2 as a prior probability, and carrying out update on basis of the step 3 and the step 4. The update is carried by adopting the Bayes' formula. The detection method of the fatigue degrees of the programmers is capable ofidentifying fatigue degree of a programmer in a human-machine system when state of the programmer is unknown but output of a keyboard and a mouse is available.