The invention discloses a convenient-to-replace cleaning brush for a glass wine cup. The cleaning brush comprises a brush rod, a mounting rod and a positioning mechanism, wherein a connecting sleeve is welded to the outer wall of the brush rod; a movable rod is mounted in the connecting sleeve; a first hairbrush roller is adhered to the top end of the movable rod; the positioning mechanism is mounted on the outer wall of the top end of the brush rod; a connecting strip is adhered to the outer side wall of the positioning mechanism; the top end of the connecting strip is connected with a firstconnecting rope and a second connecting rope; the top end of the first connecting rope is connected with a first clamp block; and the top end of the second connecting rope is connected with a second clamp block. According to the convenient-to-replace cleaning brush for the glass wine cup, by arranging the positioning mechanism, a positioning ring can clamp the whole hairbrush inside the wine cup,so that a user can conveniently rotate the hairbrush and clean the interior of the wine cup; and elastic sponge can prevent the positioning ring from directly contacting with the cup wall and has theprotective effect on the wine cup.