The invention discloses a method for accelerating and promoting demineralized dentin remineralization under the assistance of direct current, belongs to the technical field of biological material engineering, and aims to provide a method for accelerating demineralized dentin remineralization. The method comprises the following steps: S1, phosphorylating demineralized dentin tablets; S2, fixing thephosphorylated demineralized dentin tablets in the middle of a double-channel tube, putting one part of the double-channel tube into a casein phosphopeptide-nano amorphous calcium phosphate emulsion,and fixing the other part in a solution box containing simulated saliva; S3, leading out the positive electrode and the negative electrode of an electrophoresis instrument by using leads; S4, puttingan electrode connected to the negative electrode of the electrophoresis instrument into one part of a double-channel tube, allowing the tube to enter the casein phosphopeptide-nano amorphous calciumphosphate emulsion, and putting an electrode connected to the positive electrode of the electrophoresis instrument into the other part of a double-channel tube containing the simulated saliva; S5, switching on the power supply of the electrophoresis instrument to conduct electricity, and adjusting current and voltage; and S6, cleaning the dentin tablet sample obtained by the S5 so as to obtain remineralized demineralized dentin tablets.