The invention provides a harmony search algorithm for solving mixed loading of various large-batch cold-chain articles. The method comprises the following steps: firstly, acquiring various parametersof to-be-conveyed cold-chain articles and a used single-temperature-zone refrigerator car, establishing a mixed loading model when different cold-chain articles are assembled to the single-temperature-zone refrigerator car, then establishing an initial harmony memory bank, obtaining an initial solution by adopting a random search algorithm, and repairing the solution through a repairing operator until a required number of solution vectors are obtained; secondly, calculating a new solution according to the parameters and harmony search rules and repaired, and finally obtaining a cold-chain article mixed loading scheme meeting the conditions. Under the condition that the temperature constraint, the volume constraint and the weight constraint of the single-temperature-zone refrigerator car are met, a harmony search algorithm is adopted for automatic calculation, and an assembling scheme with the minimum humidity difference, the minimum shelf life difference and the minimum mixed loading type difference as targets is generated.