The invention relates to a transparent polyimide film, and a preparation method and application thereof. The general structural formula of the polyimide film is as shown in a formula (I) which is described in the invention. The transparent polyimide film provided by the invention has unique alicyclic structures and fluorine-containing fluorenyl structures, so a conjugated structure in the molecular structure of the polyimide film is effectively damaged, the charge transfer capacity of a main structure is weakened, and thermal stability is properly reserved due to introduction of the fluorenylstructure. The prepared film achieves high glass-transition temperature Tg (larger than or equal to 380 DEG C) and high light transmittance (larger than or equal to 90% at a wavelength of 550 nm). Thetransparent polyimide film can be applied to the photoelectric fields of flexible transparent display substrate materials, optical transparent films, optical communication materials, solar cell substrates and the like.