The invention discloses a preparation method of a quantum dot, a quantum dot/UV glue solution and a quantum dot doped optical fiber, and belongs to the technical field of nano materials and optical communication. According to the preparation method of a quantum dot, the Cu2ZnSnS4 quantum dot is prepared by a thermal decomposition method, and the controllable synthesis of quantum dot structure andsize can be realized, and the product has high dispersibility and uniformity and has few impurity phases. The technical problem that the pure-phase Cu2ZnSnS4 quantum dot is difficult to synthesize issolved, the quantum dot obtained can be directly used for preparing the quantum dot doped optical fiber, and great industrial value is achieved.