The invention discloses ink for tempered glass and a preparation method of the ink. The ink for the tempered glass is prepared from raw materials in parts by weight as follows: 20-35 parts of inorganic pigment, 15-35 parts of bismuth glass powder, 15-30 parts of n-tridecane, 6-10 parts of petroleum ether, 2-4 parts of a dispersing agent and 0.2-0.5 parts of anti-settling wax, wherein the bismuth glass powder is prepared from bismuth trioxide, boric acid, lithium silicate, sodium silicate, calcium oxide, phosphotungstic acid and isobutyl phenylacetate. The ink for the tempered glass can be applied to the tempered glass and has the advantage of being easy to disperse without agglomeration, and meanwhile, the preparation method has the advantages of being simple in process and simple and convenient to operate.