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发明公开
CN110317830A 一种鸭腺病毒2型DNA疫苗及其制备方法和应用 审中-实审

一种鸭腺病毒2型DNA疫苗及其制备方法和应用
摘要：
本发明一种鸭腺病毒2型DNA疫苗及其制备方法和应用，属于免疫学技术领域。所述制备方法包括：合成如SEQ ID NO.3所示的DAV-hexon序列；构建pcDNA3.1-DAV-hexon质粒；pcDNA3.1-DAV-hexon质粒转染293T细胞，在293T细胞中表达得到hexon蛋白即鸭腺病毒2型DNA疫苗。所述鸭腺病毒2型DNA疫苗由上述制备方法制得。所述鸭腺病毒2型DNA疫苗在制备预防鸭腺病毒2型疫病疫苗中的应用。本发明具有以下优点：SEQ ID NO.3所示的DAV-hexon序列有利于抗原蛋白的表达；更能提高hexon的表达量；可对番鸭提供80％-90％的攻毒保护。
摘要（英）：

The invention discloses a duck adenovirus type 2 DNA vaccine and a preparation method and application thereof and belongs to the technical field of immunology. The preparation method comprises the following steps: synthesizing a DAV-hexon sequence shown as SEQ ID NO.3; constructing a pcDNA3.1-DAV-hexon plasmid; transfecting 293T cells by the pcDNA3.1-DAV-hexon plasmid, and executing expression inthe 293T cells to obtain a hexon protein, namely the duck adenovirus type 2 DNA vaccine. The duck adenovirus type 2 DNA vaccine is prepared by the preparation method. The duck adenovirus type 2 DNA vaccine is applied to preparation of vaccine for preventing a duck adenovirus type 2 epidemic disease. The duck adenovirus type 2 DNA vaccine has the following advantages that the DAV-hexon sequence shown as SEQ ID NO.3 is conductive to the expression of an antigen protein; the expression amount of the hexon can be increased; 80%-90% of attacking protection can be provided for Muscovy ducks.

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