The invention provides a chromosome long segment insertion detection method and a long segment insertion detection method based on a MassARRAY platform, and relates to the technical field of biology.The detection method includes the steps: simultaneously identifying whether samples to be detected are wild and/or mutant by a first detection unit; identifying whether the samples to be detected aremutant or not by a second detection unit; reading and discriminating identification results of the first detection unit and identification results of the second detection unit; judging whether chromosome long segments are inserted into the samples to be detected or not. By the aid of the additional arranged second detection unit, chromosome long segment insertion can be effectively detected according to the identification results of the first detection unit and the second detection unit, false negative results are effectively decreased, and false positive results can be effectively decreased.Besides, the chromosome long segment insertion detection method has the advantage that types of the samples to be detected can be expanded.