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发明公开
CN110297083A 三嵌段纳米金的构建及其应用 无效 - 撤回

三嵌段纳米金的构建及其应用
摘要：
本发明涉及生物技术领域，具体涉及一种Poly A介导的可调控纳米金探针的构建及其应用。所述Poly A介导的可调控的纳米金探针，包括纳米金颗粒，三嵌段DNA，报告序列和两个不同的荧光基团。所述三嵌段DNA包含Poly A和两段识别序列，Poly A用于与纳米金表面结合，两段识别序列可以用于不同的生物分子识别。所述的报告序列修饰所述的荧光基团。所述荧光基团淬灭在纳米金表面。与本发明首次在Poly A两端连接识别序列，并且通过组装探针实现两种分子的检测。
摘要（英）：

The invention relates to the field of biotechnology, and particularly relates to construction and application of a Poly A-mediated regulatable and controllable nanogold probe. The Poly A-mediated regulatable and controllable nanogold probe comprises nanogold particles, triblock DNA, a reporting sequence and two different fluorophores. The triblock DNA comprises Poly A and two segments of recognition sequences, the Poly A is used for binding to the surface of the nanogold, and the two segments of recognition sequences can be used for recognizing different biomolecules. The reporting sequence modifies the fluorophores. The fluorophores are quenched on the surface of the nanogold. The recognition sequences are connected to the two ends of the Poly A in the invention for the first time, and the detection of two molecules is achieved by assembling the probe.

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