The present invention relates to a method for producing a recombinant Acremonium chrysogenum strain which produces 7-aminocephalosporanic acid at a high concentration, and to a strain produced using the method. More specifically, the present invention relates to: a method for producing an Acremonium chrysogenum strain which has a high 7-ACA production capability; a recombinant Acremonium chrysogenum strain produced using the method; a method for producing 7-ACA, wherein the strain is cultured under conditions capable of producing 7-ACA and collected; a promoter for highly expressing protein in an Acremonium chrysogenum strain used in the method for producing 7-ACA; an expression vector for highly expressing CPC acylase in an Acremonium chrysogenum strain; a use of the strain in the production of 7-ACA; a use of the produced 7-ACA in the production of an antibiotic agent; and a method for treating bacterial infectious diseases, wherein the method comprises administering the 7-ACA. According to the method of the present invention, 7-ACA can be produced at a high concentration by producing a recombinant Acremonium chrysogenum strain and fermenting the same.